COURSE TITLE

Biology 210; Genetics and Molecular Biology

PROFESSOR(S)

Jeff Knight, jknight@mtholyoke.edu
Craig Woodard, cwoodard@mtholyoke.edu

LECTURE

MTuWF, 50 Mins

LAB

3 Hour Lab Required

TEXTS

Griffiths, AJF, Gelbart, WM, Miller, JH, Lewontin, RC. 2002. Modern Genetic Analysis. 2nd ed. W. H. Freeman Company.

http://www.mtholyoke.edu/courses/cwoodard/biol210.html

EXAMS & GRADING

Please note that there are prerequisite courses that you must have completed before you enroll in this class: Biological Sciences 151, Biological Sciences 200, and Chemistry 101 or Chemistry 150. If you have not already taken these courses (or their approved equivalents), you should not be enrolled in this class. Please see Mr. Knight or Mr. Woodard if you have any questions about these prerequisites.

There will be five quizzes offered throughout the semester on the indicated dates, and each student's lowest quiz grade will be disregarded. The three midterm exams will be given on the dates listed below. The final exam will be self-scheduled.

Requirement	Point Value
MidTerm Exams (3 @ 100 each)	300
Final Exam	200
Quizzes	100
Laboratory Work (Includes 2 Major Lab Reports)	200
Total Points Available	800

LECTURE SCHEDULE

Major Topic	Textbook Reading
Introduction to Genetics	Chapter 1
The Molecular Nature of Genes The Structure of Genes and Genomes - RNA processing - Protein Structure	Chapter 2 p. 64 - 66 p. 66 - 68
Gene Function and Mutations - The molecular basis of mutation - Transposable genetic elements - Molecular basis of dominance and recessiveness	Chapters 3 & 10 p. 74 - 81, 315 - 331; 249 - 250, 289 - 290; 302, 331 - 337; 81 - 84
Molecular Biology - Concepts and Techniques - Intro. to Recombinant DNA - Making recombinant DNA - Cloning - Polymerase Chain Reaction (PCR) - Gel Electrophoresis and Southern Blotting	Chapter 8 p. 217 - 220;222 - 227;220 - 222; 230 - 233
Classical Transmission Genetics
- Mendel and the Garden Pea	p. 125 - 126
- Monohybrid Crosses (Complete Dominance, Incomplete Dominance, Lethals)	p. 117 - 125, 459 - 463
- Dihybrid Crosses	p. 150 - 156
- Sex Linkage and Inheritance Related to Sex	p. 126 - 128
- Human Genetics and Pedigree Analysis	p. 128 - 137
- Probability I (Binomial Equation)
- Probability II (the Chi-Square Test)	p. 167 - 169
- Gene Interaction - Epistasis	p. 463 - 470
- Biochemical Genetics Complementation	p. 75 - 81; 456 - 459
- Multiple Alleles
- Penetrance and Expressivity	p. 471 - 472
- Linkage and Recombination	p. 156 - 167
Genomics, and The Human Genome Project	Chapters 8 & 9
- Making and Using DNA Libraries	p. 227 - 233, and Lab Manual Project 1
- Mapping Human Genes	p. 266 - 280
- Positional Cloning	p. 232 - 234
- DNA Sequencing	p. 236 - 239
- The Human Genome Project	p. 269 - 271; 280 - 308
- Genetic Testing	p. 239 - 242
- Genetic Engineering and Gene Therapy	p. 243 - 255
Genetic and Molecular Analysis in Model Systems	Chapter 16
- Review of Drosophila Embryonic Development	p. 509 - 542
- Regulation of gene transcription	Chapter 13
- Identification of Genes Controlling Fly Embryonic Development	p. 527
- Homeotic genes, Homeoboxes, Homeodomains, and The Hox genes	p. 537 - 540; 544 - 545; 251 - 252
Genetic Phenomena in Eukaryotes
- Eukaryotic Chromosome Structure	p. 41 - 48
- Changes in chromosome number and structure	Chapter 11
- Quantitative inheritance	Chapter 18

LAB SCHEDULE

Week	Topic
1	No Lab
2	Lab Introduction and Discussion Project 1: Screen for P-elements Session 1: Transform E. coli Cells with Plasmid Containing Probe DNA, Plate Cells
3	Project 2: Mutational Analysis Session 1: Mutant Screening
4	Project 1: Screen for P-elements Session 2: Identify Transformants on Plates, Calculate Transformation Efficiencies Isolation of Fly DNA I Report Due Project 1 Introduction
5	Project 1: Screen for P-elements Session 3: Isolation of Fly DNA II, Restriction Digestion of Fly DNA
6	Project 2: Mutational Analysis Session 2: Mutant Characterization I Project 1: Screen for P-elements Session 4: Run Gel on Digested Genomic DNA and Set up Southern Blot
7	No Lab Report Due in Class Project 2 Flow Chart
8	Project 1: Screen for P-elements Session: Isolate Plasmid Containing Probe DNA from Transformed Cells Set up PCR Reaction to Prepare Probe Fragment
9	Project 1: Screen for P-elements Session 6: Run Gel on PCR Reaction and Isolate Probe DNA Fragment Project 2: Mutational Analysis Session 3: Mutant Characterization II Report Due Project 2 Introduction
10	Project 1: Screen for P-elements Session 7: Receive and Analyze Southern Blot Data
11	Project 1 Screen for P-elements Follow-Up: Discuss Southern Blot Data
12	Project 2: Mutational Analysis Session 4: Map Mutations Report Due in Class Project 1 Final Report
13	No Lab
14	Student Symposium
15	No Lab Report Due in Class Project 2 Final Report

Last Modified: March 11, 2005