COURSE TITLE Biology 220; Cell Biology PROFESSOR(S) Frank DeToma, fdetoma@mtholyoke.edu Amy Hitchcock, alhitchc@mtholyoke.edu LECTURE MW, 75 minutes and F, 50 minutes LAB 3 Hour Lab Required TEXTS Bruce Alberts et al. 2002. The Molecular Biology of the Cell, 4th ed. Garland Science Publishing Company. COURSE PREREQUISITES Biol. Sci. 145 or 150 Biol. Sci. 200 Chem. 201 or 202 EXAMS & GRADING Three one-hour exams have been pre-scheduled and will be given during LECTUREs. We expect that you will take these exams at the scheduled times. Exceptions to this rule should be rare; permission for an exception must be obtained from Ms. Hitchcock and Mr. DeToma prior to the time of the exam. The final examination will be scheduled. Quizzes or other lecture based assignments will be passed out in lecture at various scheduled points during the semester. These may or may not be assigned a grade. Performance in various class activities will be weighted as follows in assignment of final grades: Exams, quizzes, and other lecture-based assignments   65% Lab work   35% LECTURE SCHEDULE Lecture Topic 1 Introduction 2 Energetics 3 Membrane and lipid biochemistry 4 Protein and amino acid biochemistry 5 Protein primary and secondary structure 6 Protein tertiary and quarternary structure 7 Protein tertiary and quarternary structure continued 8 Membrane proteins 9 Protein production and sorting 10 Protein turnover   Exam 1 11 Nuclear protein transport 12 Nuclear protein transport continued 13 Mitochondrial and chloroplast protein transport 14 Secretory and endoplasmic reticulum protein transport 15 Overview and mechanisms of membrane transport 16 ER to Golgi protein transport 17 Lysosomal/vacuolar protein transport 18 Protein endocytosis 19 Protein exocytosis 20 Enzyme catalysis 21 Enzyme catalysis continued, enzyme kinetics   Exam 2 22 Enzyme kinetics continued 23 ATP as an energy carrier 24 Glycolysis 25 Citric acid cycle 26 Electron/proton transport in the mitochondria 27 Oxidative phosphorylation 29 Energy capture and storage in autotrophs 29 Chloroplast electron transport, photophosphoylation 30 The Calvin cycle 31 Cytoskeletal components/functions overview 31 Microtubules   Exam 3 32 Actin 33 Signal transduction: overview and common themes 34 Selected signal transduction topics 35 Selected signal transduction topics LAB SCHEDULE Week Topic 1 No Lab 2 Protein “Fingerprinting” 3 Yeast Genetics and Protein Secretion I 4 Yeast Genetics and Protein Secretion II 5 Yeast Genetics and Protein Secretion III 6 Light Microscopy 7 Fluorescence or Electron Microscopy 8 No Lab 9 Fluorescence or Electron Microscopy 10 Enzyme Kinetics 11 Mitochondrial Isolation and Assays 12 Apoptosis I 13 Apoptosis II 14 Apoptosis Presentations Last Modified: March 11, 2005

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