Biological Sciences 210 Fall, 2005 GENETICS AND MOLECULAR BIOLOGY

TENTATIVE LABORATORY SCHEDULE

Week of:

September 5 NO LABORATORY

September 12 Lab Introduction and Discussion
Project 1: Screen for P-elements Session 1: Transform E. coli Cells with Plasmid Containing Probe DNA, Plate Cells

September 19 Project 2: Mutational Analysis Session 1: Mutant Screening

September 26 Project 1: Screen for P-elements Session 2:
- Identify Transformants on Plates, Calculate Transformation Efficiencies
-Isolation of Fly DNA I

Report Due – Project 1 Introduction

October 3 Project 1: Screen for P-elements Session 3: Isolation of Fly DNA II, Restriction Digestion of Fly DNA

October 10 NO LABORATORY

October 17 Project 2: Mutational Analysis Session 2: Mutant Characterization I

Project 1: Screen for P-elements Session 4: Run Gel on Digested Genomic DNA and Set up Southern Blot

Report Due in Class, Wednesday, October 19 – Project 2 Flow Chart

October 24 Project 1: Screen for P-elements Session 5: Isolate Plasmid Containing Probe DNA from Transformed Cells
-Set up PCR Reaction to Prepare Probe Fragment

Report Due – Project 2 Introduction

October 31 Project 1: Screen for P-elements Session 6:
-Run Gel on PCR Reaction and Isolate Probe DNA Fragment

Project 2: Mutational Analysis Session 3: Mutant
Characterization II

November 7 Project 1: Screen for P-elements Session 7:
-Purify PCR product and label, set up hybridization reaction

November 14 Project 1 Screen for P-elements Follow-Up:
- Hybridize blot to antibody, develop with chemiluminescence and expose to film

November 21 NO LABORATORY

November 28 Project 2: Mutational Analysis Session 4: Map Mutations

Report Due in Class, Monday, November 28 – Project 1 Final Report

December 5 Student Symposium

December 12 NO LABORATORY

Report Due in Class, Monday, December 12 – Project 2 Final Report