Biological Sciences 210
Fall 2008

GENETICS AND MOLECULAR BIOLOGY

Instructors:

Jeff Knight, 105 Clapp, ext. 2487, jknight

Craig Woodard, 120E Carr, ext. 2724, cwoodard

TENTATIVE LABORATORY SCHEDULE

Week of

Sept. 1 - NO LABORATORY

Sept. 8 - Lab introduction and Discussion
PTC Gene Analysis, Session 1: Determination of PTC-tasting phenotype and Isolation of DNA from Cheek Cells

Sept. 15 - PTC Gene Analysis, Session 2: Set up of a Polymerase Chain Reaction (PCR) to amplify the PTC gene sequence from the extracted DNA
................Drosophila Mutational Analysis, Session 1: Screening of mutant flies

Sept. 22 - PTC Gene Analysis, Session 3: Set up of a restriction digest of the amplified PCR product (PTC gene fragment)

ASSIGNMENT DUE: Experimental plan for Drosophila mutational analysis

Sept. 29 - PTC Gene Analysis, Session 4: Separation of the restriction enzyme digest using an electrophoretic gel

Oct. 6 - PTC Gene Analysis, Session 5: Purification of the DNA from bands cut from the gel
.............Drosophila Mutational Analysis, Session 2: Characterization I of mutation

ASSIGNMENT DUE: Introduction for PTC Gene Analysis

Oct. 13 - NO LABORATORY (Fall Break)

Oct. 20 - PTC Gene Analysis, Session 6: Calculation of allele frequencies for the PTC gene based on the Hardy-Weinberg equilibrium

ASSIGNMENT DUE: Introduction for Drosophila Mutational Analysis

Oct. 27 - PTC Gene Analysis, Session 7: Analysis of sequence data using bioinformatics (BLAST searches) and discussion of results

...............Drosophila Mutational Analysis, Session 3: Characterization II of mutation

Nov. 3 - Bacterial Transformation, Session 1

Nov. 10 - Bacterial Transformation, Session 2

ASSIGNMENT DUE: PTC Gene Analysis Lab Report

Nov. 17 - Drosophila Mutational Analysis, Session 4: Mapping of mutation

Nov. 24 - NO LABORATORY (Thanksgiving Break)

Dec. 1 - Student Symposium

Dec. 8 - NO LABORATORY

ASSIGNMENT DUE: Drosophila Mutational Analysis Lab Report