Biological Sciences 210
Fall 2008
GENETICS AND MOLECULAR BIOLOGY
Instructors:
Jeff Knight, 105 Clapp, ext. 2487, jknight
Craig Woodard, 120E Carr, ext. 2724, cwoodard
Course Web Site: http://www.mtholyoke.edu/courses/cwoodard/biol210.html
There is also a course Ella site, and the course web site can be accessed
via the Ella site.
Textbook: Griffiths, et al. (2008). Introduction to Genetic Analysis, 9th Edition.
New York, W.H. Freeman and Company.
ISBN-13: 978-0-7167-6887-6
ISBN-10: 0-7167-6887-9
There will also be assigned readings, and readings available on the World Wide Web.
There will also be assigned readings, and readings available on the course
Ella site and the World Wide Web.
Please note that there are prerequisite courses that you must have completed
before you enroll in this class: Biological Sciences 145 (or 150 or 160) and
Biological Sciences 200. If you have not already taken these courses (or their
approved equivalents), you should not be enrolled in this class. Additionally,
if you have not already taken Chemistry 101 or its approved equivalent, you
must be currently enrolled in Chemistry 101 this semester. Please see Mr. Knight
or Mr. Woodard if you have any questions about these prerequisites.
The course requirements for Biology 210 are as follows. There are a total of
750 points that can be earned:
Course Requirement .............................................................................................................................Total
Points
Midterm Exams (3) ...........................................................................................................................................300
Final Exam ........................................................................................................................................................150
Quizzes .............................................................................................................................................................100
Laboratory Work (Includes 2 Major Lab Reports) (SEE
LAB SCHEDULE)......................................................200
Total points for the Course .................................................................................................................................750
There will be five quizzes offered throughout the semester on the indicated dates, and each student's lowest quiz grade will be disregarded. The three midterm exams will be given on the dates listed below. The final exam will be self-scheduled.
| Quiz Dates | Midterm Exam Dates |
|
|
TENTATIVE LECTURE SCHEDULE
Topic...................................................................................
Reading in Griffiths
Introduction to Genetics and Molecular Biology ...................Chapter
1
The Molecular Nature of Genes............................................
Chapters 8 and 9 (Much of this is review.)
The Structure of Genes and Genomes.....................
.............. 31-37, 295-309
Protein Structure .....................
.....................
.....................
321-324
Gene Function and Mutations
The molecular basis of mutation.....................
.....................
324-329, 513-518
Transposable elements.....................
.....................
...............
488-504
Molecular basis of dominance and recessiveness.....................
221-226
Molecular Biology – Concepts and Techniques .....................
Chapter 20, and Lab Manual
Polymerase Chain Reaction (PCR) .....................
.....................
731-732
Gel Electrophoresis.....................
.....................
.....................
16-17, 607-609
DNA Sequencing.....................
.....................
.....................
.. 732-735
Bioinformatics .....................
.....................
.....................
......463-468, and assigned
readings
Intro. to Recombinant DNA
Making recombinant DNA.....................
.....................
...............715-720
Cloning .....................
.....................
.....................
.....................
720-723
Classical Transmission Genetics
Mendel and the Garden Pea .....................
.....................
.............37-57
Monohybrid Crosses (Complete Dominance, Incomplete ..............223-230
Dominance, Lethals)
Dihybrid Crosses .....................
.....................
.....................
........89-94
Sex Linkage and Inheritance Related to Sex .....................
.............61-65, 71-74
Human Genetics and Pedigree Analysis .....................
.....................
66-71
Probability I (Binomial Equation).....................
.....................
....... 74-75, 94-97
Probability II (the Chi-Square Test) .....................
.....................
97-99
Gene Interaction – Epistasis.....................
.....................
............ 239-247
Biochemical Genetics
Complementation.....................
.....................
.....................
...... 230-239
Multiple Alleles
Penetrance and Expressivity.....................
.....................
............. 247-249
Linkage and Recombination.......................................... .............. 129-145
Genomes and Genomics
Introduction to Genomics .....................
.....................
..................Chapter 13
Making and using DNA libraries..................... ..................... ...... 722-730
Identifying Human Genes.....................
.....................
..................... 463-468
Human Genome Structure .....................
.....................
.....................
468-470
Comparative Genomics .....................
.....................
.....................
470-475
Functional Genomics and Reverse Genetics.....................
................ 475-482
Genetic Testing .....................
.....................
.....................
...............739-740
Genetic Engineering and Gene Therapy .....................
.....................
741-754
Genetic and Molecular Analysis in Model Systems .....................
.....Chapter 12, especially pages
415-426), 759- 773
Regulation of Gene Transcription.....................
.....................
.......... Chapters 10 and 11 (especially pages 351- 364,
and 385-402)
Genetic Phenomena in Eukaryotes
Changes in chromosome number and structure.....................
............ Chapter 11
Quantitative inheritance.......................................... ..................... .... Chapter 18
Sample Final Exam Questions (Molecular Biology)
Answers to Sample Final Exam Questions (Molecular Biology)