Courses
Biochemistry 311: Protein Biochemistry and Cellular Metabolism
Biochemistry 314: Nucleic Acids Biochemistry and Molecular Biology
Biochemsitry 330: Topics in Biochemistry
and Molecular Biology
Research
 |
Statement of Research Interest: Research in my laboratory is aimed at understanding
the role of abortive initiation and promoter escape in transcription
initiation by E. coli RNA polymerase. Previous results
indicate the extent of abortive initiation and efficiency of
promoter escape to depend on DNA sequence information embedded
in the promoter recognition region (PRR; -60 to -1) and initial
transcribed sequence (ITS; +1 to +20) of the promoter. Our work
is focused on dissecting the ITS sequence contribution, using
in vitro transcriptional analysis of promoters mutagenized
in the ITS region, and examining the pattern and extent of abortive
and productive RNA formation by high-resolution PAGE. A typical
result of the PAGE analysis, done with three phage promoters--T7
A1, T5 N25, and T5 N25anti--is shown in the gel
picture on the left. A
progress report of our work can be gleaned from the poster abstract presented
in the 1997 FASEB Summer Research Conference on Prokaryotic Transcription
Initiation. |
Publications
Current Lab Members
D. Dewaine Jackson, Ph.D.,
postdoctoral associate
Katia J. Evans, '99
Elizabeth Goneska, '99
Jillian Frechette, '00
Maureen Khoo, '99
Ruchita Sinha, '99
Undergraduate Research
Students
Service
Sponsored Research Officer, 1998-2000
Office
of the Dean of Faculty
Sponsored Research
Grants Opportunities:
MHC -- Faculty Grants
and Fellowships; Forms
External Grants: COS,
GrantsNet
Acting Chair of Chemistry
Department, 1999.
Personal
Curriculum Vita
Pictures
Useful
Links
RasMol
To Download RasMol
Expasy
Swiss Protein
Data Base
University
of Texas: World Lecture Hall
Biochemistry
Biotechnology
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