Biological Sciences 210

Fall, 2005

 

GENETICS AND MOLECULAR BIOLOGY

 

Instructors: Jeff Knight, 105 Clapp, ext. 2487, jknight@mtholyoke.edu

Amy Springer, 120B Carr, ext. 2805, aspringe@mtholyoke.edu

 

Course Web Site: http://www.mtholyoke.edu/courses/aspringe/biol210.html

 

Textbook: Griffiths, A.J.F., Gelbart, W.M., Miller, J.H., and Lewontin,

R.C. (2002). Modern Genetic Analysis: Integrating Genes and Genomes. New York, W.H. Freeman and Company, 2nd edition, ISBN: 0-7167-4382-5

 

There will also be assigned readings, and readings available on the World Wide Web.

 

Please note that there are prerequisite courses that you must have completed before you enroll in this class: Biological Sciences 150 (or 145) and Biological Sciences 200. If you have not already taken these courses (or their approved equivalents), you should not be enrolled in this class. Additionally, if you have not already taken Chemistry 101 or its approved equivalent, you must be currently enrolled in Chemistry 101 this semester. Please see Mr. Knight or Ms. Springer if you have any questions about these prerequisites.

 

The course requirements for Biology 210 are as follows. There are a total of 800 points that can be earned:

 

Course Requirement Total Points

 

Midterm Exams (3) 300

Final Exam 200

Quizzes 100

Laboratory Work (Includes 2 Major Lab Reports) 200

Total points for the Course = 800

 

There will be five quizzes offered throughout the semester on the indicated dates, and each student's lowest quiz grade will be disregarded. The three midterm exams will be given on the dates listed below. The final exam will be self-scheduled.

 

Quiz Dates (NOTE: All Midterm Exam Dates

quizzes are on Tuesdays.)

 

Tuesday, September 20

Tuesday, September 27 Wednesday, October 5

Tuesday, October 18 Wednesday, November 2

Tuesday, November 8 Wednesday, December 7

Tuesday, November 15

TENTATIVE LECTURE SCHEDULE

 

Topic Reading in Griffiths

Introduction to Genetics Chapter 1

 

The Molecular Nature of Genes

The Structure of Genes and Genomes Chapter 2

Eukaryotic Chromosome Structure 41-48

RNA processing 64-66

Protein Structure 66-68

 

Gene Function and Mutations Chapters 3 and 10

The molecular basis of mutation 74-81, 315-331

Transposable genetic elements 249-250, 289-290, 302, 331-337

 

Molecular basis of dominance and recessiveness 81-84

 

Molecular Biology Concepts and Techniques

Intro. to Recombinant DNA Chapter 8

Making recombinant DNA 217-220

Cloning 222-227

Polymerase Chain Reaction (PCR) 220-222

Gel Electrophoresis and Southern Blotting 230-233

 

Classical Transmission Genetics

Mendel and the Garden Pea 125-126

 

Monohybrid Crosses (Complete Dominance, Incomplete 117-125, 459-463

Dominance, Lethals)

 

Dihybrid Crosses 150-156

 

Sex Linkage and Inheritance Related to Sex 126-128

 

Human Genetics and Pedigree Analysis 128-137

 

Probability I (Binomial Equation)

 

Probability II (the Chi-Square Test) 167-169

 

Gene Interaction Epistasis 463-470

 

Biochemical Genetics 75-81

Complementation 456-459

 

Multiple Alleles

Penetrance and Expressivity 471-472

 

Linkage and Recombination 156-167

 

 

 

 

 

Genomics, and The Human Genome Project Chapters 8 and 9

Making and Using DNA Libraries 227-233, and Lab Manual Project 1

 

Mapping Human Genes 266-280

Positional Cloning 232-234

DNA Sequencing 236-239

The Human Genome Project 269-271, 280-308

Genetic Testing 239-242

Genetic Engineering and Gene Therapy 243-255

 

Genetic and Molecular Analysis in Model Systems Chapter 16

Review of Drosophila Embryonic Development 509-542

Regulation of gene transcription Chapter 13

 

Identification of Genes Controlling Fly Embryonic Development 527

 

Homeotic genes, Homeoboxes, Homeodomains, and The Hox genes 537-540, 544-545

251-252

 

Genetic Phenomena in Eukaryotes

Changes in chromosome number and structure Chapter 11

 

Quantitative inheritance Chapter 18