1970 Douglass College, B.A., Biology
1975 University of Michigan, Ph.D., Biological Chemistry
HONORS AND AWARDS
1970 Phi Beta Kappa
1971-3 Barbour Scholar, University of Michigan
1975-7 PHS National Research Service Award
1982 Sigma Xi
1982-4 Research Corp., "Studies of E. coli tRNA gene regulation in vitro and in vivo"
1985-7 NIH-AREA, "E. coli ArgRS represses tRNAArg gene expression in vitro", $75,280.
1986-8 NSF-CSIP, "Modernization of an upper-level laboratory experience in biochemistry"
1988-90 NIH-AREA, "Regulation studies of the E. coli argT promoter", $87,450.
1992-3 NSF-VPW to U.C. Berkeley, "Mechanistic investigation of transcription initiation and
elongation by E. coli RNA polymerase", $104,000.
1993-5 NIH-AREA, "Investigation of the mechanism of transcription initiation by E. coli RNA
1994 NSF-ARI, "Acquisition of a phosphor imaging analysis work station," $50,400.
1997-00 NIH-AREA, "Analysis of the determinants of promoter clearance", $107,400.
1997-00 NIH minority supplement for undergraduate student, $26,335.
1975-6 Postdoctoral Fellow, Univ. of Michigan (with William R. Folk)
1976-8 Postdoctoral Fellow, Roche Inst. of Mol. Biol. (with James Ofengand)
1978-81 Research Associate, Univ. of Massachusetts (with Maurille J. Fournier)
1987 Sabbatical Researcher, U.C. Berkeley (with Michael J. Chamberlin)
1992-3 Visiting Assoc. Professor, U.C. Berkeley (with Michael J. Chamberlin)
1981-87 Assistant Professor of Biochemistry, Mount Holyoke College
1987-94 Associate Professor of Biochemistry, Mount Holyoke College
1991-97 Chair, Program in Biochemistry, Mount Holyoke College
1992-93 Visiting Associate Professor, University of California at Berkeley
1994- Professor of Biochemistry, Mount Holyoke College
1997-00 Sponsored Research Officer, Mount Holyoke College
1998-99 Acting Chair of Chemistry
Special Reviewer, NIH Study Section MBC-2, 11/88, 10/90; MBC-1, 10/91; MBC-2, 6/98
Reviewer, NSF-ILI Grant Review Panel, February, l993
Member, NIH Study Section MBC-1, October, l993 - June, 1997
Reviewer for the AAAS-Merck Grants, January, 1997
Refereed journal articles for PNAS and JMB
RECENT (POSTER) PRESENTATIONS
(*indicates undergraduate co-authors)
Hsu, L. M., *Bao, Y., *Xia, L., *Ahn, H., and *Carroll, K. S. "Initial Transcribed Sequence
and Promoter Escape," a poster presented at the FASEB Summer Research Conference on
Transcription Initiation in Prokaryotes, July 19-24, 1997, Saxtons River, Vermont.
*Evans, K. J. and Hsu, L. M. "RNA Polymerase Open Complex Stability and Abortive Cycling:
Role of the Discriminator Sequence in transcription Initiation," a poster presented at the
American Chemical Society National Meeting, August 22-27, 1998, Boston, MA.
PUBLICATIONS (* indicates undergraduate co-author)
|Ofengand, J., Lin, F.-L., Hsu, L.,
Keren-Zur, M., & Boublik, M. (l980) Topology of the
ribosomal sites for tRNA as revealed by photoaffinity labeling. Annals N. Y. Acad. Sci.
|Ofengand, J., Lin, F.-L., Hsu, L.,
& Boublik, M. (l981) Three-dimensional arrangement of
tRNA at the donor and acceptor sites of the E. coli ribosome in Molecular Approaches to Gene Expression and Protein Structure (Siddiqui, M. A. Q., Krauskopf, M., and
Weissbach, H., eds.), pp. 1-31, Academic Press, New York.
|Hsu, L. M., Lin, F.-L., Nurse, K., & Ofengand, J. (1984) Covalent crosslinking ofEscherichia coli phenylalanyl-tRNA and valyl-tRNA to the ribosomal A site via photoaffinity probes attached to the 4-thiouridine residue. J. Mol. Biol. 172, 57-76.|
|Hsu, L. M., Klee, H. J., Zagorski, J., & Fournier, M. J. (1984) Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine and proline tRNAs. J. Bacteriol. 158, 934-942.|
|Hsu, L. M., Zagorski, J., & Fournier, M. J. (1984) Cloning and sequence analysis of the Escherichia coli 4.5S RNA gene. J. Mol. Biol. 178, 509-531.|
|Hsu, L. M., Zagorski, J., Wang, Z., & Fournier, M. J. (1985) Escherichia coli 6S RNA gene is part of a dual function transcription unit. J. Bacteriol. 161, 1162-1170.|
|Hsu, L. M., *Giannini, J. K., Leung, T.-W. C., & Crosthwaite, J. C. (l99l) Upstream sequence activation of E. coli argT promoter in vivo and in vitro. Biochemistry 30, 813-22.|
|Hsu, L. M., Vo, N. V., & Chamberlin, M. J. (1995) Escherichia coli transcript cleavage factors GreA and GreB stimulate promoter clearance andgGene expression in vivo and in vitro. Proc. Natl. Acad. Sci. USA 92, 11588-11592.|
|Hsu, L. M. (1996) Quantitative parameters for promoter clearance. Meth. Enzymol. 273, 59-71.|
|Chamberlin, M. J. & Hsu, L.
M. (1996) RNA chain initiation and promoter escape by RNA
polymerase. In Regulation of Gene Expression in Escherichia coli, E. C. C. Lin & A. S.
Lynch, eds., R. G. Landes Company, Austin, pp. 7-25.
|Hernandez, V. J., Hsu, L. M., & Cashel, M. (1996) Conserved region 3 of Escherichia coli sigma 70 is implicated in the process of abortive transcription. J. Biol. Chem. 271, 18775-18779.|
|Hsu, L. M. & Chamberlin, M. J. (1998) Studies of RNA chain initiation, abortive initiation, and promoter escape by Escherihchia coli RNA polymerase at three bacteriophage promoters. J. Mol. Biol., accepted for publication pending revision.|
UNDERGRADUATE RESEARCH STUDENTS:
Undergraduate research students (1993-1998): total = 25
Current list of undergraduate research students: 5; Katia J. Evans, '99; Ruchita Sinha, '99;
Maureen Khoo, '99; Elizabeth Goneska, '99; and Jillian R. Frechette, '00.