Mount Holyoke College

In vivo cytoskeletal dynamics of living fish embryos
Movie #2:Deep cell circus movements: Actin dynamics


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In this movie you can watch a cell undergoing sequential blebbing--a form of protrusion where the cell membrane locally "lets go" of the actin meshwork underlying it. Fluid pressure in the cell leads to a herniation, and a hemispheric protrusion called a bleb is formed. When these blebs spread "sideways" along the surface of the cell, it is called circus movements. To translocate, the cell pours the rest of the cell body into the protrusion, and then blebs again at the front end. These deep cells are migrating through the yolk sac of a killifish embryo. The brightly labeled actin cortex can be followed in this GFP-actin labeled cell. When blebbing occurs, the cortex is briefly left behind, and then is reorganized under the new front end of the cell. This cell happens to have two nuclei (the two round, black objects in the cytoplasm).