Mount Holyoke College

Fish Keratocyte Locomotion

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(Two movies on this page)

When a scale is plucked from a killifish (Fundulus heteroclitus), it is surrounded by thin layers of tissue. Hilary Genise peeled the attached tissue off of the scale, and cultured it between two coverslips. In her time-lapse video, you can see individual cells begin to migrate away from the large mass of tissue. Events are speeded up 120 times.

The movie above is a higher magnification view of keratocyte locomotion, and was made by Hanna Dastigir and Vonnie Lee. Here you can see individual cells move using large, ruffling lamellipodia. Events are speeded up 48 times.